Anti-Apolipoprotein CIII抗体(ab21032)
Key features and details
- Rabbit polyclonal to Apolipoprotein CIII
- Suitable for: ICC/IF, Sandwich ELISA, ELISA, WB
- Reacts with: Human, Monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Apolipoprotein CIII抗体
参阅全部 Apolipoprotein CIII 一抗 -
描述
兔多克隆抗体to Apolipoprotein CIII -
宿主
Rabbit -
特异性
Specifically binds to human Apo CIII. -
经测试应用
适用于: ICC/IF, Sandwich ELISA, ELISA, WBmore details -
种属反应性
与反应: Human, Monkey -
免疫原
Full length protein corresponding to Human Apolipoprotein CIII. Native protein
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.0292% EDTA, 0.435% Sodium chloride -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified by Human Apo CIII-Sepharose affinity column. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
ELISA pair antibody
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21032于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
Sandwich ELISA |
Use at an assay dependent concentration. PubMed: 21371998
|
|
ELISA |
1/5000 - 1/40000. Can be paired for ELISA with Biotin Goat polyclonal to Apolipoprotein CIII (ab21024).
|
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
|
说明 |
---|
ICC/IF
Use a concentration of 5 µg/ml. |
Sandwich ELISA
Use at an assay dependent concentration. PubMed: 21371998 |
ELISA
1/5000 - 1/40000. Can be paired for ELISA with Biotin Goat polyclonal to Apolipoprotein CIII (ab21024). |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
靶标
-
功能
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
组织特异性
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
疾病相关
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
序列相似性
Belongs to the apolipoprotein C3 family. -
翻译后修饰
O-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
-
别名
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
图片
-
All lanes : Anti-Apolipoprotein CIII antibody (ab21032) at 1 µg/ml
Lane 1 : Native Human Apolipoprotein CIII (ab77944) at 0.1 µg
Lane 2 : Native Human Apolipoprotein CIII (ab77944) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1 minute -
ICC/IF image of ab21032 stained HepG2 (Human liver hepatocellular carcinoma cell line) cells.
The cells were fixed in 4% formaldehyde for 10 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21032, 5 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa-Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa-Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour.
DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab21032 被引用在 5 文献中.
- Yang M et al. Alantolactone suppresses APOC3 expression and alters lipid homeostasis in L02 liver cells. Eur J Pharmacol 828:60-66 (2018). PubMed: 29555504
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Schjoldager KT et al. Probing isoform-specific functions of polypeptide GalNAc-transferases using zinc finger nuclease glycoengineered SimpleCells. Proc Natl Acad Sci U S A 109:9893-8 (2012). IP ; Human . PubMed: 22566642
- Swarup V et al. Identification and quantification of differentially expressed proteins in plasma of spinocerebellar ataxia type 12. Neurosci Res 73:161-7 (2012). PubMed: 22426495
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus monkey . PubMed: 21371998