概述

  • 产品名称Anti-Apolipoprotein CII抗体
    参阅全部 Apolipoprotein CII 一抗
  • 描述
    兔多克隆抗体to Apolipoprotein CII
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Apolipoprotein CII .

    (Peptide available as ab88220.)

  • 阳性对照
    • This antibody gave a positive signal in Human Plasma Total Protein Lysate. IHC-P: human normal liver FFPE tissue sections

相关产品

应用

Our Abpromise guarantee covers the use of ab76452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 11 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 相关性At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-II (apoCII) is in found in chylomicrons (large lipoprotein particles absorbed from the gastrointestinal tract) and VLDL (large lipoproteins that are broken down to eventually form LDL). ApoCII activates the enzyme lipoprotein lipase, which hydrolyzes triglycerides and thus provides free fatty acids for cells.
  • 细胞定位Secreted
  • 数据库链接
  • 别名
    • APC 2 antibody
    • APC2 antibody
    • Apo CII antibody
    • APOC 2 antibody
    • ApoC II antibody
    • APOC2 antibody
    • APOC2 protein antibody
    • APOC2_HUMAN antibody
    • ApoCII antibody
    • Apolipoprotein C II antibody
    • Apolipoprotein C II precursor antibody
    • Apolipoprotein C2 antibody
    • ApolipoproteinCII antibody
    • MGC75082 antibody
    see all

Anti-Apolipoprotein CII antibody 图像

  • Anti-Apolipoprotein CII antibody (ab76452) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 11 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • ICC/IF image of ab76452 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76452, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml
  • All lanes : Anti-Apolipoprotein CII antibody (ab76452) at 1 µg/ml

    Lane 1 : Human Apolipoprotein CII full length protein (ab77878) at 0.1 µg
    Lane 2 : Human Apolipoprotein CII full length protein (ab77878) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes
  • IHC image of Apolipoprotein CII staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76452, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Apolipoprotein CII antibody (ab76452)参考文献

This product has been referenced in:
  • Okubo M  et al. Apolipoprotein C-II Tuzla: a novel large deletion in APOC2 caused by Alu-Alu homologous recombination in an infant with apolipoprotein C-II deficiency. Clin Chim Acta 438:148-53 (2015). WB ; Human . Read more (PubMed: 25172036) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Aug 20 2015

Application Western blot
Loading amount 5 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (Isoelectrofocusing pH4-6.5)
Sample Human Serum (d<1.006 fraction isolated by ultracentrifuge)
Specification d<1.006 fraction isolated by ultracentrifuge
Treatment dissolved in 8M Urea
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

実 大久保

Verified customer

提交于 Sep 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"