Anti-Apolipoprotein CI抗体(ab20793)

概述

  • 产品名称Anti-Apolipoprotein CI抗体
    参阅全部 Apolipoprotein CI 一抗
  • 描述
    兔多克隆抗体to Apolipoprotein CI
  • 特异性Ab20793 specifically binds to human Apo CI. No cross-reactions with Human Apo AI, Apo AII, Apo B-100, Apo CII, Apo CIII, or Apo E.
  • 经测试应用适用于: ICC/IF, Indirect ELISA, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human Apolipoprotein-CI).

应用

Our Abpromise guarantee covers the use of ab20793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 mg/ml.
Indirect ELISA Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 9 kDa.
ELISA 1/5000 - 1/20000. Dilution optimised using Chromogenic detection.

靶标

  • 功能Appears to modulate the interaction of APOE with beta-migrating VLDL and inhibit binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • 组织特异性Synthesized mainly in liver and to a minor degree in intestine. Secreted in plasma.
  • 序列相似性Belongs to the apolipoprotein C1 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APO C1 antibody
    • Apo CI antibody
    • Apo-CIB antibody
    • Apo-CIB' antibody
    • APOC 1 antibody
    • ApoC I antibody
    • ApoC-IB antibody
    • ApoC-IB' antibody
    • APOC1 antibody
    • APOC1_HUMAN antibody
    • APOC1B antibody
    • Apolipoprotein C I antibody
    • Apolipoprotein C I variant I antibody
    • Apolipoprotein C-I antibody
    • Apolipoprotein C1 antibody
    • Apolipoprotein CI antibody
    • ApolipoproteinC I antibody
    • ApolipoproteinCI antibody
    • Truncated apolipoprotein C-I antibody
    see all

Anti-Apolipoprotein CI antibody 图像

  • ICC/IF image of ab20793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20793, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Apolipoprotein CI antibody (ab20793)参考文献

ab20793 has not yet been referenced specifically in any publications.

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The antibody has been used to detect Apolopoprotein CI in an indirect ELISA. This was performed using theantige...

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