概述

  • 产品名称Anti-Apolipoprotein B抗体
    参阅全部 Apolipoprotein B 一抗
  • 描述
    山羊多克隆抗体to Apolipoprotein B
  • 特异性This antibody has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins.
    Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards.
    Non-specific cross-reaction of anti-apolipoprotein antibodies with other Human serum proteins is negligible.
  • 经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native Human Apolipoprotein B isolated from Human plasma.

  • 阳性对照
    • Human liver tissue

性能

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应用

Our Abpromise guarantee covers the use of ab98132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 516 kDa.
IP Use at an assay dependent concentration.
ELISA 1/1000 - 1/5000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
EMSA Use at an assay dependent concentration.

靶标

  • 功能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 疾病相关Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 序列相似性Contains 1 vitellogenin domain.
  • 翻译后修饰Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody 图像

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human liver tissue labelled with ab98132 at 5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab98132 staining Apolipoprotein B in Mouse liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer pH6.0. Samples were incubated with primary antibody (0.5 ug/ml in BSA/TBS buffer) for 30 minutes. ab97101 Rabbit polyclonal Secondary Antibody to Anti-goat HRP, IgG - H&L, pre-adsorbed (1/500) was used as the secondary antibody.

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Anti-Apolipoprotein B antibody (ab98132)参考文献

This product has been referenced in:

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Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Protein Block as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization No
Blocking step Protein Block as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"