Anti-Apolipoprotein B抗体(ab7616)


  • 产品名称Anti-Apolipoprotein B抗体
    参阅全部 Apolipoprotein B 一抗
  • 描述
    山羊多克隆抗体to Apolipoprotein B
  • 特异性Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA. This antibody reacts with human apoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apoLipoproteins.
  • 经测试应用适用于: Dot blot, ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rabbit, Cow, Human
  • 免疫原

    Full length native apoLipoprotein Type B (purified).

  • 阳性对照
    • ICC/IF: HepG2 cells.
  • 常规说明

    This antibody has been used to determine that atherosclerotic lesions in the human aorta contain considerable amounts of lipoproteins. These lipoproteins were observed to be complexed with components of the extracellular matrix (especially LDL and proteoglycans). The role of these matrix-lipoprotein complexes is not entirely clear, however, animal models of atherosclerosis have shown that increased cellular proliferation and increased production of extracellular matrix components occur following injury to the intimal layer of the aorta.

    At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Abcam's apo-lipoproteins are derived from human plasma by density gradient ultracentrifugation and HPLC.



Our Abpromise guarantee covers the use of ab7616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot 1/5000 - 1/10000.
ELISA 1/2000 - 1/10000.
IHC-P 1/50 - 1/200.
WB 1/200 - 1/1000.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 疾病相关Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 序列相似性Contains 1 vitellogenin domain.
  • 翻译后修饰Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody 图像

  • ab7616 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab7616 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Apolipoprotein B antibody (ab7616)参考文献

This product has been referenced in:
  • Shimizu M  et al. Quercetin represses apolipoprotein B expression by inhibiting the transcriptional activity of C/EBPß. PLoS One 10:e0121784 (2015). WB ; Human . Read more (PubMed: 25875015) »
  • Liu L  et al. Effects of nonesterified fatty acids on the synthesis and assembly of very low density lipoprotein in bovine hepatocytes in vitro. J Dairy Sci 97:1328-35 (2014). Cow . Read more (PubMed: 24377799) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Nov 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization No
Specification Liver
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Nov 10 2015

Thank you for contacting us.

The gel that was used was Tris-Acetate. We use Invitrogen's NuPAGE 3-8% TA gels:
I hope this information is helpful to you. Please do not hesitate to co...

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Thank you for contacting us.
I have contacted the lab asking for the protocol used to produce the WB shown on the datasheet. Here is the answer I got:
Ab77859 was used at 0.01 ug as shown in the WB. When we used the protein at higher concentr...

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Vielen Dank für Ihren Anruf.
Tatsächlich hat eine meiner Kolleginnen mit der Turbidimetrie gearbeitet, und nach einiger Diskussion sind wir der Meinung, dass für Ihren Kunden Antikörper, die in ELISA und/oder IP funktionieren, geeignet sein sollte...

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We have not mapped the epitope for ab7616 and have not tested the antibody against Apo B48 and B100. We have no information about the cross reactivity to Apo B48 and B100.

I am sorry for not being...

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Thank you for getting back to us with further details. I am handling Charlottes enquiries in her absence. Once again I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been having with this anti...

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Thank you for your enquiry. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognise a different epitope. I am not sure that the antibody can be used to prevent the Apo B100 antibody from...

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Thank you for your enquiry. When ab7616 was tested for application in Western blotting, it was tested against purified Apo B protein, which would be recommended as a positive control. If you have any additional questions, please contact us again.

Thank you for your email. Ab7616 was made against the whole apo B and should be able to recognize all the residues. I'm not familiar with the antibody from Polymedco, so unfortunately can't state how this antibody works in comparison. If you have any m...

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