Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
应用说明IHC-P: see figure below
This antibody gave a positive result in ELISA against the immunizing peptide (ab50068).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
疾病相关Hypobetalipoproteinemia, familial, 1 Familial ligand-defective apolipoprotein B-100 Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
序列相似性Contains 1 vitellogenin domain.
翻译后修饰Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
This Fast-Track antibody is not yet fully characterised. These images represent
inconclusive preliminary data.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Apolipoprotein B antibody (ab50069)
IHC image of Apoliporotein B staining in human liver carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50069, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation - Anti-Apolipoprotein B antibody (ab50069)This image is courtesy of an anonymous abreview.
Immunoprecipitation of Apolipoprotein B, using a Huh7.5 human hepatoma cell line conditioned medium. The protein was immunoprecipitated from 1.8 mL spent medium, using 29µg of Rabbit polyclonal to Apolipoprotein B (ab50069). A different Apolipoprotein B antibody was used for the western blot.
Lanes 1: Pull down with ab50069, Lane 2: IP control with rabbit IgG.