概述

  • 产品名称Anti-Apolipoprotein B抗体
    参阅全部 Apolipoprotein B 一抗
  • 描述
    兔多克隆抗体to Apolipoprotein B
  • 特异性ab31992 recognizes Apolipoprotein B48 and B100, as these proteins are highly conserved.
  • 经测试应用适用于: WB, Immunodiffusionmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    SDS PAGE purified mouse Apolipoprotein B48 (very low density lipoprotein) from pooled mouse plasma.

应用

Our Abpromise guarantee covers the use of ab31992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
Immunodiffusion
  • 应用说明Radial Immunodiffusion (RID): 1/10 - 1/25.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 516 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • 疾病相关Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • 序列相似性Contains 1 vitellogenin domain.
    • 翻译后修饰Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • 细胞定位Cytoplasm. Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apo B-100 antibody
      • Apo B-48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    Anti-Apolipoprotein B antibody (ab31992)参考文献

    This product has been referenced in:
    • Johnson LA  et al. Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism and vascular inflammation. J Lipid Res 54:386-96 (2013). Mouse . Read more (PubMed: 23204275) »
    • Maczuga P  et al. Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther : (2012). Read more (PubMed: 23089734) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (liver, brain)
    Loading amount 15 µg
    Specification liver, brain
    Gel Running Conditions Reduced Denaturing
    Blocking step 5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 11 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - other (Mouse C57/BL6 EDTA plasma)
    Loading amount 30 µg
    Specification Mouse C57/BL6 EDTA plasma
    Treatment starving O/N
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Andreas Gille

    Verified customer

    提交于 Feb 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"