Anti-Apolipoprotein B抗体[12G10] (ab63960)


  • 产品名称
    Anti-Apolipoprotein B抗体[12G10]
    参阅全部 Apolipoprotein B 一抗
  • 描述
    小鼠单克隆抗体[12G10] to Apolipoprotein B
  • 特异性
    ab63960 reacts with LDL of human plasma. It is specific to the T4 fragment of Apo-B (residues 1-1297). No crossreactions with other human plasma proteins were detected.
  • 经测试应用
    适用于: WB, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human LDL

  • 阳性对照
    • This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.


Our Abpromise guarantee covers the use of ab63960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 515 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • 疾病相关
    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • 序列相似性
    Contains 1 vitellogenin domain.
  • 翻译后修饰
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • 细胞定位
    Cytoplasm. Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody [12G10] 图像

  • ab63960 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63960 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%) HeLa and HepG2 cells, and also in formaldehyde fixed (4%) HeLa, HepG2, and MCF-7 cells at 5ug/ml.

Anti-Apolipoprotein B antibody [12G10] (ab63960)参考文献

ab63960 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am very pleased to hear you would like to make use of our AbTrial offer and test ab63960 in Mouse cells with ICC/IF.

Expiration date: 30th March 2013

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Thank you for contacting us.

Unfortunately ab63960 has not been tested in any applications other than WB and Flow Cytometry. It doesn’t mean it won’t work in other applications, but we don’t have data to support it.
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