RabMAb

Anti-Apolipoprotein A II抗体[EPR2913] (ab92478)

概述

  • 产品名称Anti-Apolipoprotein A II抗体[EPR2913]
    参阅全部 Apolipoprotein A II 一抗
  • 描述
    兔单克隆抗体[EPR2913] to Apolipoprotein A II
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apolipoprotein A II.

  • 阳性对照
    • Human plasma lysate. HEPG2 cells; rat primary astrocytes,
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

应用

Our Abpromise guarantee covers the use of ab92478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • 组织特异性Plasma; synthesized in the liver and intestine.
    • 序列相似性Belongs to the apolipoprotein A2 family.
    • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APO A2 antibody
      • Apo AII antibody
      • Apo-AII antibody
      • APOA 2 antibody
      • ApoA II antibody
      • ApoA-II antibody
      • APOA2 antibody
      • APOA2_HUMAN antibody
      • APOAII antibody
      • Apolipoprotein A II antibody
      • Apolipoprotein A-II(1-76) antibody
      • Apolipoprotein A2 antibody
      • Apolipoprotein AII antibody
      • ApolipoproteinA II antibody
      • OTTHUMP00000032244 antibody
      see all

    Anti-Apolipoprotein A II antibody [EPR2913] 图像

    • Anti-Apolipoprotein A II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. 

      See Abreview

    • Ab92478 at 1/100 dilution staining Apolipoprotein A II in HepG2 cells by immunofluorescence.

    Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)参考文献

    ab92478 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary astrocyte culture)
    Permeabilization Yes - 0.1M PBS with 1% Triton X
    Specification Primary astrocyte culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Methanol
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Sep 06 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"