Anti-Apolipoprotein A II抗体[EPR2913] (ab92478)


  • 产品名称Anti-Apolipoprotein A II抗体[EPR2913]
    参阅全部 Apolipoprotein A II 一抗
  • 描述
    兔单克隆抗体[EPR2913] to Apolipoprotein A II
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apolipoprotein A II.

  • 阳性对照
    • Human plasma lysate. HEPG2 cells; rat primary astrocytes,
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Our Abpromise guarantee covers the use of ab92478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/500.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
    • 组织特异性Plasma; synthesized in the liver and intestine.
    • 序列相似性Belongs to the apolipoprotein A2 family.
    • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APO A2 antibody
      • Apo AII antibody
      • Apo-AII antibody
      • APOA 2 antibody
      • ApoA II antibody
      • ApoA-II antibody
      • APOA2 antibody
      • APOA2_HUMAN antibody
      • APOAII antibody
      • Apolipoprotein A II antibody
      • Apolipoprotein A-II(1-76) antibody
      • Apolipoprotein A2 antibody
      • Apolipoprotein AII antibody
      • ApolipoproteinA II antibody
      • OTTHUMP00000032244 antibody
      see all

    Anti-Apolipoprotein A II antibody [EPR2913] 图像

    • Anti-Apolipoprotein A II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma lysate at 10 µg

      HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size : 11 kDa
      Observed band size : 11 kDa
    • Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody. 

      See Abreview

    • Ab92478 at 1/100 dilution staining Apolipoprotein A II in HepG2 cells by immunofluorescence.

    Anti-Apolipoprotein A II antibody [EPR2913] (ab92478)参考文献

    ab92478 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Primary astrocyte culture)
    Permeabilization Yes - 0.1M PBS with 1% Triton X
    Specification Primary astrocyte culture
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Methanol

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Sep 06 2016