重组Anti-Apolipoprotein A II/ApoA-II抗体[EPR2913] (ab92478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2913] to Apolipoprotein A II/ApoA-II
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Apolipoprotein A II/ApoA-II抗体[EPR2913]
参阅全部 Apolipoprotein A II/ApoA-II 一抗 -
描述
兔单克隆抗体[EPR2913] to Apolipoprotein A II/ApoA-II -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human Apolipoprotein A II/ApoA-II aa 50-150. The exact sequence is proprietary.
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阳性对照
- Human plasma lysate. HEPG2 cells; rat primary astrocytes,
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2913 -
同种型
IgG -
研究领域
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab92478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
|
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ICC/IF | (1) |
1/100 - 1/500.
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说明 |
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WB
1/1000 - 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
ICC/IF
1/100 - 1/500. |
靶标
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功能
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
组织特异性
Plasma; synthesized in the liver and intestine. -
序列相似性
Belongs to the apolipoprotein A2 family. -
翻译后修饰
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 336 Human
- Entrez Gene: 25649 Rat
- Omim: 107670 Human
- SwissProt: P02652 Human
- SwissProt: P04638 Rat
- Unigene: 237658 Human
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别名
- APO A2 antibody
- Apo AII antibody
- Apo-AII antibody
see all
图片
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Anti-Apolipoprotein A II/ApoA-II antibody [EPR2913] (ab92478) at 1/1000 dilution + Human plasma
lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Immunocytochemistry/Immunofluorescence analysis rat primary astrocyte culture labelling Apolipoprotein A II/ApoA-II with ab92478 at 1/500. Cells were fixed with methanol and permeabilised using 0.1M PBS with 1% Triton X. An Alexa Fluor® 488-conjugated monoclonal donkey antibody at 1/3000 dilution was used as the secondary antibody.
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Ab92478 at 1/100 dilution staining Apolipoprotein A II/ApoA-II in HepG2 cells by immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab92478 被引用在 4 文献中.
- Kobayashi T et al. Glycation of HDL Polymerizes Apolipoprotein M and Attenuates Its Capacity to Bind to Sphingosine 1-Phosphate. J Atheroscler Thromb 28:730-741 (2021). PubMed: 32999208
- Zhao L et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). PubMed: 31351426
- May-Zhang LS et al. Modified sites and functional consequences of 4-oxo-2-nonenal adducts in HDL that are elevated in familial hypercholesterolemia. J Biol Chem 294:19022-19033 (2019). PubMed: 31666337
- Holzer M et al. HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants. J Lipid Res 58:2220-2228 (2017). PubMed: 28893842