概述

  • 产品名称Anti-Apolipoprotein A II抗体
    参阅全部 Apolipoprotein A II 一抗
  • 描述
    兔多克隆抗体to Apolipoprotein A II
  • 经测试应用适用于: WB, ELISA, Conjugation, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human Apolipoprotein A II.

  • 阳性对照
    • IF/ICC: HepG2 cell line. IHC-P: Liver FFPE tissue sections.

应用

Our Abpromise guarantee covers the use of ab24241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/5000 - 1/40000. Predicted molecular weight: 11 kDa.
ELISA 1/5000 - 1/40000.
Conjugation Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.
  • 组织特异性Plasma; synthesized in the liver and intestine.
  • 序列相似性Belongs to the apolipoprotein A2 family.
  • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APO A2 antibody
    • Apo AII antibody
    • Apo-AII antibody
    • APOA 2 antibody
    • ApoA II antibody
    • ApoA-II antibody
    • APOA2 antibody
    • APOA2_HUMAN antibody
    • APOAII antibody
    • Apolipoprotein A II antibody
    • Apolipoprotein A-II(1-76) antibody
    • Apolipoprotein A2 antibody
    • Apolipoprotein AII antibody
    • ApolipoproteinA II antibody
    • OTTHUMP00000032244 antibody
    see all

Anti-Apolipoprotein A II antibody 图像

  • ICC/IF image of ab24241 stained HepG2 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24241, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC image of Apolipoprotein A II staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24241, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Apolipoprotein A II antibody (ab24241)参考文献

This product has been referenced in:
  • Tomazic PV  et al. Nasal mucus proteomic changes reflect altered immune responses and epithelial permeability in patients with allergic rhinitis. J Allergy Clin Immunol N/A:N/A (2013). WB ; Human . Read more (PubMed: 24290289) »

See 1 Publication for this product

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