概述

  • 产品名称Anti-ApoER2抗体[EPR3326]
    参阅全部 ApoER2 一抗
  • 描述
    兔单克隆抗体[EPR3326] to ApoER2
  • 经测试应用适用于: WB, IPmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ApoER2 aa 950 to the C-terminus (C terminal).
    Database link: Q14114
    (Peptide available as ab193569)

  • 阳性对照
    • SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab108208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 106 kDa.
IP 1/10 - 1/100.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
    • 组织特异性Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
    • 疾病相关Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
    • 序列相似性Belongs to the LDLR family.
      Contains 2 EGF-like domains.
      Contains 7 LDL-receptor class A domains.
      Contains 5 LDL-receptor class B repeats.
    • 结构域The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
    • 翻译后修饰O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
      Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
      Tyrosine phosphorylated upon apoE binding.
      Ubiquitinated by MYLIP leading to degradation.
    • 细胞定位Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
    • Information by UniProt
    • 数据库链接
    • 别名
      • APOER2 antibody
      • Apolipoprotein E receptor 2 antibody
      • low density lipoprotein receptor-related protein 8 antibody
      • Low-density lipoprotein receptor-related protein 8 antibody
      • LRP-8 antibody
      • LRP8 antibody
      • LRP8_HUMAN antibody
      see all

    Anti-ApoER2 antibody [EPR3326] 图像

    • All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)

      Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : C6 (rat glioma) whole cell lysate
      Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 106 kDa
      Observed band size : 130-170 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer 5% NFDM/TBST.

      130kDa: Immature form;

      170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)

    • All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : Human Fetal Brain cell lysate
      Lane 5 : Mouse Fetal Brain cell lysate
      Lane 6 : Rat Fetal Brain cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 106 kDa
    • ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate 10ug

      Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-ApoER2 antibody [EPR3326] (ab108208)参考文献

    This product has been referenced in:
    • Brai E  et al. Notch1 Regulates Hippocampal Plasticity Through Interaction with the Reelin Pathway, Glutamatergic Transmission and CREB Signaling. Front Cell Neurosci 9:447 (2015). WB, IHC-FoFr ; Mouse . Read more (PubMed: 26635527) »
    • Neman J  et al. Human breast cancer metastases to the brain display GABAergic properties in the neural niche. Proc Natl Acad Sci U S A 111:984-9 (2014). Human . Read more (PubMed: 24395782) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Human THP-1 cell line, various mouse tissues)
    Loading amount 15 µg
    Specification Human THP-1 cell line, various mouse tissues
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel (Novex))
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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    提交于 Nov 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"