概述

  • 产品名称Anti-ApoER2抗体
    参阅全部 ApoER2 一抗
  • 描述
    兔多克隆抗体to ApoER2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human ApoER2 aa 928-945 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    GEPRSQLHQLPKNPLSEL

  • 阳性对照
    • HEK-293T cells expressing human ApoER2.

性能

应用

Our Abpromise guarantee covers the use of ab62659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 110 kDa.

靶标

  • 功能Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
  • 组织特异性Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
  • 疾病相关Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
  • 序列相似性Belongs to the LDLR family.
    Contains 2 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • 结构域The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
  • 翻译后修饰O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
    Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
    Tyrosine phosphorylated upon apoE binding.
    Ubiquitinated by MYLIP leading to degradation.
  • 细胞定位Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APOER2 antibody
    • Apolipoprotein E receptor 2 antibody
    • low density lipoprotein receptor-related protein 8 antibody
    • Low-density lipoprotein receptor-related protein 8 antibody
    • LRP-8 antibody
    • LRP8 antibody
    • LRP8_HUMAN antibody
    see all

Anti-ApoER2 antibody 图像

  • All lanes : Anti-ApoER2 antibody (ab62659) at 1/2000 dilution

    Lane 1 : HEK293 cells co-transfected with human ApoER2
    Lane 2 : wt HEK293 cells (untransfected)
    Lane 3 : Inhibition with ApoER2 (human 928-945) peptide (20 µg/mL)


    Predicted band size : 110 kDa

Anti-ApoER2 antibody (ab62659)参考文献

ab62659 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62659.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"