概述

  • 产品名称Anti-ApoER2抗体
    参阅全部 ApoER2 一抗
  • 描述
    小鼠单克隆抗体to ApoER2
  • 经测试应用适用于: WB, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment, corresponding to amino acids 83-171 of Human ApoER2

性能

应用

Our Abpromise guarantee covers the use of ab58216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. (methanol fixed cells)



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
  • 组织特异性Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
  • 疾病相关Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
  • 序列相似性Belongs to the LDLR family.
    Contains 2 EGF-like domains.
    Contains 7 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • 结构域The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
  • 翻译后修饰O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
    Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
    Tyrosine phosphorylated upon apoE binding.
    Ubiquitinated by MYLIP leading to degradation.
  • 细胞定位Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APOER2 antibody
    • Apolipoprotein E receptor 2 antibody
    • low density lipoprotein receptor-related protein 8 antibody
    • Low-density lipoprotein receptor-related protein 8 antibody
    • LRP-8 antibody
    • LRP8 antibody
    • LRP8_HUMAN antibody
    see all

Anti-ApoER2 antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab58216 ApoER2 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa
  • Overlay histogram showing PC12 cells stained with ab58216 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58216, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in PC12 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-ApoER2 antibody (ab58216)参考文献

This product has been referenced in:
  • Allen KL  et al. A novel pathway for human endothelial cell activation by antiphospholipid/anti-ß2 glycoprotein I antibodies. Blood 119:884-93 (2012). Read more (PubMed: 22106343) »
  • Yang XV  et al. Activated protein C ligation of ApoER2 (LRP8) causes Dab1-dependent signaling in U937 cells. Proc Natl Acad Sci U S A 106:274-9 (2009). WB ; Human . Read more (PubMed: 19116273) »

See all 2 Publications for this product

Product Wall

Application Flow Cytometry
Fixation no fixing
Permeabilization No
Sample Human Cell (HEK 293 cells transfected with ApoEr2 Isoforms)
Specification HEK 293 cells transfected with ApoEr2 Isoforms
Gating Strategy intact cellular bodies
Preparation Cell harvesting/tissue preparation method: Cell Surface Indirect Labelling
Sample buffer: PBS
Username

Dr. Henry King

Verified customer

提交于 Nov 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK 203 cells transfected with ApoEr2 Isoforms)
Specification HEK 203 cells transfected with ApoEr2 Isoforms
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Henry King

Verified customer

提交于 Aug 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK)
Specification HEK
Fixative Formaldehyde
Permeabilization No
Blocking step (agent) for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Henry King

Verified customer

提交于 Apr 08 2013

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (Fc-ectodomain)
Loading amount 1 µg
Specification Fc-ectodomain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (THP-1 cell line)
Specification THP-1 cell line
Preparation Cell harvesting/tissue preparation method: Cells were taken from suspension culture and washed with PBS/BSA before fixation
Sample buffer: 0.1% BSA in PBS
Fixation Methanol
Permeabilization No
Gating Strategy Cell population was gated by FSC and SSC
Username

Abcam user community

Verified customer

提交于 Oct 26 2012

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