概述

  • 产品名称Apocynin
  • 描述
    Selective NADPH-oxidase inhibitor
  • 生物学描述Selective NADPH-oxidase inhibitor (IC50 = 10 μM). Inhibits production of reactive oxygen species. Also elicits a range of in vitro and in vivo anti-inflammatory effects.
  • 纯度> 99%

性能

    应用

    Our Abpromise guarantee covers the use of ab120615 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Apocynin 图像

    • ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.

    Apocynin (ab120615)参考文献

    This product has been referenced in:
    • Genovese T  et al. Modulation of NADPH oxidase activation in cerebral ischemia/reperfusion injury in rats. Brain Res 1372:92-102 (2011). Read more (PubMed: 21138737) »
    • Impellizzeri D  et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochem Pharmacol 81:636-48 (2011). Read more (PubMed: 21147071) »
    • Impellizzeri D  et al. Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. Free Radic Res 45:221-36 (2011). Read more (PubMed: 20954833) »

    See all 2 Publications for this product

    Product Wall

    Thank you very much for your call today and for your question about ab120615.

    I've looked through some literature and references, and apocynin does seem to reversibly inhibitNADPH oxidase:

    http://www.hindawi.com/journals/mi/2008/106...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"