Anti-APOBEC3G/A3G抗体(ab194581)
Key features and details
- Rabbit polyclonal to APOBEC3G/A3G
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-APOBEC3G/A3G抗体
参阅全部 APOBEC3G/A3G 一抗 -
描述
兔多克隆抗体to APOBEC3G/A3G -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat, Human
预测可用于: Gorilla -
免疫原
Recombinant fragment corresponding to Human APOBEC3G/A3G aa 50-350.
Database link: Q9HC16 -
阳性对照
- WB: A-549, Jurkat, HeLa and PC-3 cell lysates; Rat lung and spleen tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194581于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 46 kDa.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 46 kDa. |
靶标
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功能
DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing. -
组织特异性
Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. -
序列相似性
Belongs to the cytidine and deoxycytidylate deaminase family. -
翻译后修饰
Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF. - Information by UniProt
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数据库链接
- Entrez Gene: 101137376 Gorilla
- Entrez Gene: 200316 Human
- Entrez Gene: 60489 Human
- Omim: 607113 Human
- SwissProt: Q694C1 Gorilla
- SwissProt: Q9HC16 Human
- Unigene: 659809 Human
- Unigene: 660143 Human
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别名
- A3G antibody
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
see all
图片
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All lanes : Anti-APOBEC3G/A3G antibody (ab194581) at 1/1000 dilution
Lane 1 : A-549 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC-3 cell lysate
Lane 5 : Rat lung tissue lysate
Lane 6 : Rat speen tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L)
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 3 minutesBlocking buffer: 3% nonfat dry milk in TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab194581 被引用在 1 文献中.
- Chen G et al. Evaluation of CD4+ cells infiltration as a prognostic factor in cervical intraepithelial neoplasia 2. J Gynecol Oncol 34:e2 (2023). PubMed: 36245223