概述

  • 产品名称Anti-APOBEC3G抗体
    参阅全部 APOBEC3G 一抗
  • 描述
    山羊多克隆抗体to APOBEC3G
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    C-DEHSQDLSGRLR

    , corresponding to C terminal amino acids 365-376 of Human APOBEC3G (NP_068594.1).

  • 阳性对照
    • Daudi cell lysate, Human kidney and Human testis tissues

性能

应用

Our Abpromise guarantee covers the use of ab109727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
IHC-P Use a concentration of 2.5 µg/ml.

靶标

  • 功能DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
  • 组织特异性Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines.
  • 序列相似性Belongs to the cytidine and deoxycytidylate deaminase family.
  • 翻译后修饰Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
  • 细胞定位Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A3G antibody
    • ABC3G_HUMAN antibody
    • APOBEC related cytidine deaminase antibody
    • APOBEC related protein antibody
    • APOBEC-related cytidine deaminase antibody
    • APOBEC-related protein 9 antibody
    • APOBEC-related protein antibody
    • APOBEC3G antibody
    • Apolipoprotein B editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic subunit 3G antibody
    • apolipoprotein B mRNA editing enzyme cytidine deaminase antibody
    • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like antibody
    • ARCD antibody
    • ARP-9 antibody
    • ARP9 antibody
    • bK150C2.7 antibody
    • CEM-15 antibody
    • CEM15 antibody
    • deoxycytidine deaminase antibody
    • dJ494G10.1 antibody
    • DNA dC dU editing enzyme APOBEC 3G antibody
    • DNA dC->dU editing enzyme antibody
    • DNA dC->dU-editing enzyme APOBEC-3G antibody
    • EC 3.5.4. antibody
    • FLJ12740 antibody
    • MDS019 antibody
    • OTTHUMP00000028911 antibody
    • phorbolin-like protein antibody
    • phorbolin-like protein MDS019 antibody
    see all

Anti-APOBEC3G antibody 图像

  • Anti-APOBEC3G antibody (ab109727) at 0.5 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg/ml
    Developed using the ECL technique

    Predicted band size : 46 kDa
  • ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G in Human Kidney, Formalin-Fixed, Paraffin-Embedded section.
  • ab109727 at a concentration of 2.5 µg/ml staining APOBEC3G in Human Testis, Formalin-Fixed, Paraffin-Embedded section.

Anti-APOBEC3G antibody (ab109727)参考文献

ab109727 has not yet been referenced specifically in any publications.

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