Anti-APOA1BP抗体(ab75114)

概述

  • 产品名称Anti-APOA1BP抗体
    参阅全部 APOA1BP 一抗
  • 描述
    兔多克隆抗体to APOA1BP
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human APOA1BP

  • 阳性对照
    • Extracts from HeLa cells. A549 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 32 kDa.
ELISA 1/40000.
ICC/IF 1/500 - 1/1000.

靶标

  • 相关性APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I.
  • 细胞定位Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation
  • 数据库链接
  • 别名
    • AI BP antibody
    • AIBP antibody
    • ApoA I binding protein antibody
    • Apolipoprotein A I binding protein antibody
    • MGC119143 antibody
    • MGC119144 antibody
    • MGC119145 antibody
    • NAD(P)H hydrate epimerase antibody
    • NAD(P)HX epimerase antibody
    • YjeF N terminal domain containing protein 1 antibody
    • yjeF_N1 antibody
    • YJEFN1 antibody
    see all

Anti-APOA1BP antibody 图像

  • All lanes : Anti-APOA1BP antibody (ab75114) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HeLa cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-APOA1BP antibody (ab75114)参考文献

ab75114 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Multiple cell lines)
Gel Running Conditions Reduced Denaturing
Loading amount 70 µg
Specification Multiple cell lines
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jun 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"