Anti-APMAP抗体(ab81176)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to APMAP
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide within N terminal amino acids 72-121 (FKEPPLLLGV LHPNTKLRQA ERLFENQLVG PESIAHIGDV MFTGTADGRV) of Human APMAP (NP_065392)

  • 阳性对照
    • Fetal liver lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

靶标

  • 功能
    Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.
  • 组织特异性
    Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found in the liver, placenta and heart. Found on the cell surface of monocytes. In embryonic tissue, the highest expression levels is found in the liver and the kidney.
  • 序列相似性
    Belongs to the strictosidine synthase family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adipocyte plasma membrane associated protein antibody
    • Adipocyte plasma membrane-associated protein antibody
    • apmap antibody
    • APMAP_HUMAN antibody
    • BSCv antibody
    • BSCv protein antibody
    • C20orf3 antibody
    • Chromosome 20 open reading frame 3 antibody
    • Protein BSCv antibody
    see all

Anti-APMAP antibody 图像

  • Anti-APMAP antibody (ab81176) at 1 µg/ml + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-APMAP antibody (ab81176)参考文献

ab81176 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver lysate)
Gel Running Conditions
Reduced Denaturing (NUPAGE 4-12% BT GEL 1.5MM)
Loading amount
20 µg
Specification
Liver lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Jul 04 2017

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