概述

  • 产品名称
    Anti-APM2抗体
  • 描述
    兔多克隆抗体to APM2
  • 经测试应用
    适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from C terminal region of human APM2 protein Peptide available as ab95393.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Ammonium Sulphate Precipitation
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. Used succesfully in ELISA using ab95393 APM2 Peptide to create the standard curve.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See the review submitted by anonymous on 21 Jul 2010.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 8 kDa.Can be blocked with Human APM2 peptide (ab95393).

靶标

  • 相关性
    APM2 gene is exclusively expressed in adipose tissue. Its function is currently unknown. APM2 has been shown to be associated with cisplatin resistance in cell lines. It is not found in cisplatin sensitive cell lines, implicating APM2 in the development of cisplatin resistance in tumor cells.
  • 数据库链接
  • 别名
    • Adipose most abundant gene transcript 2 protein antibody
    • Adipose specific 2 antibody
    • APM 2 antibody
    • C10orf116 antibody
    • Chromosome 10 open reading frame 116 antibody
    • OTTHUMP00000020019 antibody
    • RP11-96C23.4 antibody
    see all

Anti-APM2 antibody 图像

  • ab79579 detecting APM2 in Human serum by indirect ELISA. Samples were blocked with 5% BSA for 1 hour at 26°C and were incubated with the primary antibody (1/1000) for 3 hours at 26°C. An HRP-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. ab95393 (APM2 Peptide) was used for establishing the standard curve.

    See Abreview

Anti-APM2 antibody (ab79579)参考文献

ab79579 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Type
Indirect
Username

Abcam user community

Verified customer

提交于 Sep 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Specification
Liver
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jul 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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