概述

  • 产品名称Anti-APLF抗体
    参阅全部 APLF 一抗
  • 描述
    兔多克隆抗体to APLF
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Macaque Monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human APLF.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain tissue lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab105446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

靶标

  • 功能Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
  • 序列相似性Belongs to the APLF family.
    Contains 1 FHA-like domain.
    Contains 2 PBZ-type zinc fingers.
  • 结构域The PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites.
    The FHA-like domain mediates interaction with XRCC1 and XRCC4.
  • 翻译后修饰Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
  • 细胞定位Nucleus. Cytoplasm > cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2010301N04Rik antibody
    • AI452191 antibody
    • Aplf antibody
    • APLF_HUMAN antibody
    • Aprataxin and PNK-like factor antibody
    • Apurinic-apyrimidinic endonuclease APLF antibody
    • C2orf13 antibody
    • PNK and APTX like FHA protein antibody
    • PNK and APTX-like FHA domain-containing protein antibody
    • RGD1565557 antibody
    • XIP1 antibody
    • XRCC1 interacting protein 1 antibody
    • XRCC1-interacting protein 1 antibody
    see all

Anti-APLF antibody 图像

  • Anti-APLF antibody (ab105446) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ICC/IF image of ab105446 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105446, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-APLF antibody (ab105446)参考文献

ab105446 has not yet been referenced specifically in any publications.

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