概述

  • 产品名称Anti-APH1a抗体
    参阅全部 APH1a 一抗
  • 描述
    兔多克隆抗体to APH1a
  • 特异性Recognises both isoforms of APH1a (encoded on chromosome 1)
  • 经测试应用适用于: IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from residues 50 - 150 of Human APH1a.

    .

  • 阳性对照
    • This antibody gave a positive signal in human and mouse brain lysates in WB and in mouse brain tissue sections in IF.

性能

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应用

Our Abpromise guarantee covers the use of ab12104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
  • 组织特异性Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
  • 序列相似性Belongs to the APH-1 family.
  • 细胞定位Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6530402N02Rik antibody
    • AL138795.3 antibody
    • Anterior Pharynx Defective 1 antibody
    • Anterior pharynx defective 1 homolog A antibody
    • APH 1A antibody
    • Aph 1alpha antibody
    • APH-1a antibody
    • Aph-1alpha antibody
    • Aph1a antibody
    • APH1A gamma secretase subunit antibody
    • APH1A_HUMAN antibody
    • CGI 78 antibody
    • CGI78 antibody
    • Gamma secretase subunit APH 1A antibody
    • Gamma Secretase Subunit APH1a antibody
    • Gamma-secretase subunit APH-1A antibody
    • Likely ortholog of C. elegans anterior pharynx defective 1A antibody
    • Presenilin Stabilization Factor antibody
    • Presenilin-stabilization factor antibody
    • PSF antibody
    • UNQ579/PRO1141 antibody
    see all

Anti-APH1a antibody 图像

  • All lanes : Anti-APH1a antibody (ab12104) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 57 kDa,67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab12104 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ab12104 staining APH1a in mouse brain tissue section (cortex) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. ab12104 produces a very pretty staining in neuronal processes in the cortex and the hippocampus.

    See Abreview

  • All lanes : Anti-APH1a antibody (ab12104) at 1/500 dilution

    Lane 1 : Brain (Human) tissue lysate
    Lane 2 : Brain (Mouse) tissue lysate
    Lane 3 : Brain (Human) tissue lysate with Human APH1a peptide (ab12394) at 1 µg/ml
    Lane 4 : Brain (Mouse) tissue lysate with Human APH1a peptide (ab12394) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa (possible isoform),75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-APH1a antibody (ab12104)参考文献

This product has been referenced in:
  • Tanveer R  et al. The Endocannabinoid, Anandamide, Augments Notch-1 Signaling in Cultured Cortical Neurons Exposed to Amyloid-ß and in the Cortex of Aged Rats. J Biol Chem 287:34709-21 (2012). WB ; Rat . Read more (PubMed: 22891244) »
  • Donmez G  et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . Read more (PubMed: 20655472) »

See all 2 Publications for this product

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I am happy to help. Please let me know if you have any further questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
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Dr. Sophie Pezet

Verified customer

提交于 Feb 08 2009

We have received the following information from the source of ab16555: This protein is an integral membrane protein (Potential). It is predominantly located in the endoplasmic reticulum and in the cis-Golgi. Tissue specificity: Widely expressed. Ex...

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