The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at a concentration of 1 - 2 µg/ml using human U87 cell lysates. 2.5-5.0 mL is
recommended using biotin/ExtrAvidin Peroxidase staining of heat-retrieved formalin-fixed paraffin embedded tissue from sections of human brain.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
组织特异性Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
序列相似性Belongs to the ATG7 family.
结构域The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
翻译后修饰Acetylated by EP300.
细胞定位Cytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Lanes 1 - 2 : Anti-Apg7 antibody (ab58735) at 1 µg/ml Lane 3 : Anti-Apg7 antibody (ab58735) at 2 µg/ml
Lane 1 : Whole mouse 3T3 cell extract Lane 2 : Whole mouse 3T3 cell extract with Apg7 immunizing peptide (human, 38-50) Lane 3 : Whole mouse 3T3 cell extract
Secondary HRP conjugated Goat anti-Rabbit IgG Developed using the ECL technique
Predicted band size : 78 kDa
Anti-Apg7 antibody (ab58735)参考文献
This product has been referenced in:
Hao Y et al. Lin28a protects against postinfarction myocardial remodeling and dysfunction through Sirt1 activation and autophagy enhancement. Biochem Biophys Res Commun479:833-840 (2016).
Read more (PubMed: 27693787) »