概述

  • 产品名称Anti-Apg7抗体
    参阅全部 Apg7 一抗
  • 描述
    兔多克隆抗体to Apg7
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    YRLDEAPKDIKGY

    conjugated to KLH via a C-terminal cysteine residue, corresponding to amino acids 38-50 of Human Apg7

  • 阳性对照
    • WB:whole extracts of rat PC12 or mouse 3T3 cells. IP: human U87 cell lysates, human brain sections.

相关产品

应用

Our Abpromise guarantee covers the use of ab58735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at a concentration of 1 - 2 µg/ml using human U87 cell lysates. 2.5-5.0 mL is
    recommended using biotin/ExtrAvidin Peroxidase staining of heat-retrieved formalin-fixed paraffin embedded tissue from sections of human brain.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
    • 组织特异性Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
    • 序列相似性Belongs to the ATG7 family.
    • 结构域The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
      The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
    • 翻译后修饰Acetylated by EP300.
    • 细胞定位Cytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1810013K23Rik antibody
      • Apg 7 antibody
      • APG7 autophagy 7 like antibody
      • APG7 autophagy 7-like (S. cerevisiae) antibody
      • APG7 like antibody
      • APG7, S. cerevisiae, homolog of antibody
      • APG7-like antibody
      • APG7L antibody
      • ATG 7 antibody
      • ATG12-activating enzyme E1 ATG7 antibody
      • ATG7 antibody
      • ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
      • ATG7 autophagy related 7 homolog antibody
      • ATG7_HUMAN antibody
      • Atg7l antibody
      • Autophagy 7, S. cerevisiae, homolog of antibody
      • Autophagy related protein 7 antibody
      • Autophagy-related 7 (yeast) antibody
      • Autophagy-related protein 7 antibody
      • DKFZp434N0735 antibody
      • GSA 7 antibody
      • GSA7 antibody
      • hAGP7 antibody
      • Ubiquitin activating enzyme E1 like protein antibody
      • Ubiquitin-activating enzyme E1-like protein antibody
      • Ubiquitin-like modifier-activating enzyme ATG7 antibody
      see all

    Anti-Apg7 antibody 图像

    • Lanes 1 - 2 : Anti-Apg7 antibody (ab58735) at 1 µg/ml
      Lane 3 : Anti-Apg7 antibody (ab58735) at 2 µg/ml

      Lane 1 : Whole mouse 3T3 cell extract
      Lane 2 : Whole mouse 3T3 cell extract with Apg7 immunizing peptide (human, 38-50)
      Lane 3 : Whole mouse 3T3 cell extract

      Secondary
      HRP conjugated Goat anti-Rabbit IgG
      Developed using the ECL technique

      Predicted band size : 78 kDa

    Anti-Apg7 antibody (ab58735)参考文献

    This product has been referenced in:
    • Hao Y  et al. Lin28a protects against postinfarction myocardial remodeling and dysfunction through Sirt1 activation and autophagy enhancement. Biochem Biophys Res Commun 479:833-840 (2016). WB ; Mouse . Read more (PubMed: 27693787) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"