- 数据表
- 具体的参考文献 (18)
- 实验方案
概述
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产品名称Anti-Apg7抗体
参阅全部 Apg7 一抗 -
描述兔多克隆抗体to Apg7
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宿主Rabbit
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经测试应用适用于: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
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种属反应性与反应: Mouse, Chicken, Human
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免疫原
A 17 amino acid synthetic peptide from near the C-terminus of human APG7.
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阳性对照
- Caco-2 cell lysate, MCF7 cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab53255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 21784844 | |
| ICC/IF | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa). | |
| IHC-P | Use at an assay dependent concentration. PubMed: 20174468 |
靶标
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功能E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
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组织特异性Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
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序列相似性Belongs to the ATG7 family.
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结构域The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates. -
翻译后修饰Acetylated by EP300.
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细胞定位Cytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
- Information by UniProt
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数据库链接
- Entrez Gene: 415961 Chicken
- Entrez Gene: 10533 Human
- Entrez Gene: 74244 Mouse
- Omim: 608760 Human
- SwissProt: Q5ZKY2 Chicken
- SwissProt: O95352 Human
- SwissProt: Q9D906 Mouse
- Unigene: 38032 Human
see all -
别名
- 1810013K23Rik antibody
- Apg 7 antibody
- APG7 autophagy 7 like antibody
see all
图片
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Western blot - Anti-Apg7 antibody (ab53255)Lane 1 : Anti-Apg7 antibody (ab53255) at 0.5 µg/ml
Lane 2 : Anti-Apg7 antibody (ab53255) at 1 µg/ml
Lane 3 : Anti-Apg7 antibody (ab53255) at 2 µg/ml
All lanes : Caco-2 cell lysate at 15 µg per lane.
Predicted band size: 78 kDa
Observed band size: 78 kDa -
Immunocytochemistry - Anti-Apg7 antibody (ab53255)This image shows ab53255 staining MCF7 cells as 10µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apg7 antibody (ab53255)IHC image of ab53255 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53255, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Apg7 antibody (ab53255)Image from Meng Q & Cai D, J Biol Chem. 2011 Sep 16;286(37):32324-32. Epub 2011 Jul 22. Fig 4.; doi: 10.1074/jbc.M111.254417; September 16, 2011, The Journal of Biological Chemistry, 286, 32324-32332.Immunohistochemical analysis of murine brain tissue, staining Apg7 with ab53255. Fixed brain sections were blocked with serum, permeabilized with 0.2% Triton X-100, and treated with primary antibody. An AlexaFluor®555-conjugated anti-rabbit IgG was used as the secondary antibody.
实验方案
文献
This product has been referenced in:
- Sun S et al. ATG7 promotes the tumorigenesis of lung cancer but might be dispensable for prognosis predication: a clinicopathologic study. Onco Targets Ther 9:4975-81 (2016). IHC-P ; Human . Read more (PubMed: 27563251) »
- Liu L et al. Baclofen mediates neuroprotection on hippocampal CA1 pyramidal cells through the regulation of autophagy under chronic cerebral hypoperfusion. Sci Rep 5:14474 (2015). WB . Read more (PubMed: 26412641) »
客户评价及客户问答
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