• 产品名称Anti-APG5L/ATG5抗体
    参阅全部 APG5L/ATG5 一抗
  • 描述
    鸡多克隆抗体to APG5L/ATG5
  • 特异性Three isoforms of APG5L/ATG5 are known to exist; ab106551 will only detect the longest isoform.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the N terminus of Human APG5L/ATG5 (EAW48415).

  • 阳性对照
    • Mouse spleen tissue lysate; Human spleen tissue.


Our Abpromise guarantee covers the use of ab106551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 32 kDa.


  • 功能Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
    May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
  • 组织特异性Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
  • 序列相似性Belongs to the ATG5 family.
  • 翻译后修饰Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
    Acetylated by EP300.
  • 细胞定位Cytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG 5 antibody
    • APG 5L antibody
    • APG5 antibody
    • APG5 autophagy 5 like antibody
    • APG5 like antibody
    • APG5-like antibody
    • APG5L antibody
    • Apoptosis specific protein antibody
    • Apoptosis-specific protein antibody
    • ASP antibody
    • ATG 5 antibody
    • Atg5 antibody
    • ATG5 autophagy related 5 homolog antibody
    • ATG5_HUMAN antibody
    • Autophagy protein 5 antibody
    • hAPG5 antibody
    • Homolog of S Cerevisiae autophagy 5 antibody
    • OTTHUMP00000040507 antibody
    see all

Anti-APG5L/ATG5 antibody 图像

  • Lane 1 : Anti-APG5L/ATG5 antibody (ab106551) at 1 µg/ml
    Lane 2 : Anti-APG5L/ATG5 antibody (ab106551) at 2 µg/ml

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 32 kDa
  • ab106551, at 2.5µg/ml, staining APG5L/ATG5 in Human spleen tissue by Immunohistochemistry.

Anti-APG5L/ATG5 antibody (ab106551)参考文献

ab106551 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call today and for your questions about our APG5L/ATG5 antibodies.

Have you found any further information regarding the sequence of the cleaved protein (e.g. whether it is an N-terminal or C-terminal fragment)? I have not f...

Read More