概述

  • 产品名称Anti-Apg3抗体[EPR4802]
    参阅全部 Apg3 一抗
  • 描述
    兔单克隆抗体[EPR4802] to Apg3
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues on the C terminal in Human Apg3

  • 阳性对照
    • K562, HeLa or Jurkat cell lysates (WB) Permeabilized Jurkat cells (FC)
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

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应用

Our Abpromise guarantee covers the use of ab108282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC/IF or IHC-P.
  • 靶标

    • 功能E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
    • 组织特异性Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
    • 序列相似性Belongs to the ATG3 family.
    • 翻译后修饰Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610016C12Rik antibody
      • Apg 3 antibody
      • APG3 antibody
      • APG3 autophagy 3 like antibody
      • APG3 like antibody
      • APG3, S. cerevisiae, homolog of antibody
      • APG3-like antibody
      • APG3L antibody
      • Apg3p antibody
      • ATG 3 antibody
      • ATG3 antibody
      • ATG3 autophagy related 3 homolog antibody
      • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
      • ATG3_HUMAN antibody
      • Autophagy 3, S. cerevisiae, homolog of antibody
      • Autophagy Apg3p/Aut1p like antibody
      • autophagy related 3 antibody
      • Autophagy related protein 3 antibody
      • Autophagy-related protein 3 antibody
      • DKFZp564M1178 antibody
      • FLJ22125 antibody
      • hApg3 antibody
      • MGC15201 antibody
      • OTTHUMP00000214547 antibody
      • OTTHUMP00000214548 antibody
      • PC3 96 antibody
      • Protein PC3-96 antibody
      • Ubiquitin-like-conjugating enzyme ATG3 antibody
      see all

    Anti-Apg3 antibody [EPR4802] 图像

    • Flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).
    • All lanes : Anti-Apg3 antibody [EPR4802] (ab108282) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Anti-Apg3 antibody [EPR4802] (ab108282)参考文献

    This product has been referenced in:
    • He G  et al. Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury. Apoptosis 21:390-403 (2016). WB ; Rat . Read more (PubMed: 26882903) »

    See 1 Publication for this product

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