RabMAb

Anti-Apg12抗体[EPR4799] - Autophagosome Marker (ab109492)

概述

  • 产品名称Anti-Apg12抗体[EPR4799] - Autophagosome Marker
    参阅全部 Apg12 一抗
  • 描述
    兔单克隆抗体[EPR4799] to Apg12 - Autophagosome Marker
  • 经测试应用适用于: ICC, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Apg12.

  • 阳性对照
    • THP1, HepG2, Raji, and Human fetal kidney lysate; Apg12-transfected 293T lysates; Human colon tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Apg12 antibody (Alexa Fluor® 488) - Autophagosome Marker [EPR4799] (ab185028)

性能

应用

Our Abpromise guarantee covers the use of ab109492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the ATG12 family.
  • 结构域Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Anti-Apg12 antibody [EPR4799] - Autophagosome Marker 图像

  • Overlay histogram showing HepG2 cells stained with ab109492 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109492, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilution

    Lane 1 : THP1 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Raji lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Lower band shows Apg12.
    Upper band shows Apg12-ATG5 conjugate.
  • All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilution

    Lane 1 : Apg12-transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ab109492 at 1/50 dilution staining Apg12 in paraffin embedded Human colon tissue.

Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492)参考文献

ab109492 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293 cell)
Specification HEK293 cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 16°C
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提交于 Apr 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"