概述

  • 产品名称Anti-Apg12抗体
    参阅全部 Apg12 一抗
  • 描述
    兔多克隆抗体to Apg12
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant full length protein (S. cerevisiae).

  • 阳性对照
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.

性能

应用

Our Abpromise guarantee covers the use of ab4752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/1000 - 1/5000.
    WB: 1/2000. Detects a band of approximately 22.1 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
  • 靶标

    • 功能Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the ATG12 family.
    • 结构域Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 别名
      • APG12-like antibody
      • APG12L antibody
      • ATG12 antibody
      • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
      • ATG12 autophagy related 12 homolog antibody
      • ATG12_HUMAN antibody
      • Autophagy 12 antibody
      • Autophagy-related protein 12 antibody
      • FBR93 antibody
      • HAPG12 antibody
      • Ubiquitin-like protein ATG12 antibody
      see all

    Anti-Apg12 antibody 图像

    • ab4752 generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125). Panel C shows specific reaction with APG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.

    Anti-Apg12 antibody (ab4752)参考文献

    This product has been referenced in:
    • Crews L  et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . Read more (PubMed: 20174468) »

    See 1 Publication for this product

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    Sorry but there has been no further information on this and we do not know if it will cross react with human.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"