概述

  • 产品名称Anti-Apelin抗体
    参阅全部 Apelin 一抗
  • 描述
    兔多克隆抗体to Apelin
  • 特异性This antibody reacts specifically with 8,5 kDa of human APLN protein.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Monkey
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide derived from C terminal region of human Apelin

性能

应用

Our Abpromise guarantee covers the use of ab59469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 9 kDa.
IHC-P 1/500.

靶标

  • 功能Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
  • 组织特异性Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
  • 序列相似性Belongs to the apelin family.
  • 翻译后修饰Several active peptides may be produced by proteolytic processing of the peptide precursor.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AGTRL1 ligand antibody
    • APEL antibody
    • APEL_HUMAN antibody
    • Apelin-13 antibody
    • APJ endogenous ligand antibody
    • Apln antibody
    • XNPEP2 antibody
    see all

Anti-Apelin antibody 图像

  • ab59469 (1:500) staining Apelin in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secreted regions of cells in the basal uterine glands.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Apelin antibody (ab59469)参考文献

This product has been referenced in:

See all 5 Publications for this product

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