The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells.
组织特异性Isoform 1 is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 is predominantly expressed in the aorta. Isoform 3 appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC in vivo. In response to vascular injuries ASMC dedifferentiate and change from a quiescent and contractile phenotype to a proliferative and synthetic phenotype. This proliferation of vascular smooth muscle cells is one of the most prominent features of atherosclerosis.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 2 fibronectin type-III domains. Contains 9 Ig-like (immunoglobulin-like) domains. Contains 2 protein kinase domains.
翻译后修饰May be autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.