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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apc7.
(Peptide available as ab13734.)
Our Abpromise guarantee covers the use of ab4171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
Western blot using ab4171 against Hela Nuclear Extract.
The expected band size for Apc7 is 63 kd, which corresponds to the band seen in this western. The band is blocked by the immunising peptide.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000)
Exposure time: 1min
Lane 1 and 2: 20
Lane 1: ab4171 (1/500)
Lane 2: ab4171 (1/500) + 1.0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"