The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 72, 90 kDa (predicted molecular weight: 72 kDa).
Use at 2-5 µg/mg of lysate.
功能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the APC6/CDC16 family. Contains 7 TPR repeats.
翻译后修饰Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
CDC16 homolog antibody
CDC16 protein antibody
Cell division cycle 16 antibody
Cell division cycle 16 homolog antibody
Cell division cycle protein 16 homolog antibody
Cyclosome subunit 6 antibody
Anti-Apc6 antibody 图像
Western blot - Apc6 antibody (ab72136)
All lanes : Anti-Apc6 antibody (ab72136) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 15 µg Lane 2 : Whole cell lysate from Hela cells at 5 µg Lane 3 : Whole cell lysate from Hela cells at 1.5 µg Lane 4 : Whole cell lysate from Hela cells at 0.5 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Predicted band size : 72 kDa Observed band size : 72 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Apc6 antibody (ab72136)
Immunoprecipitation/ Western Blot of Apc6.
Lane 1: ab72136 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
ab72136 at 0.1µg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection:Chemiluminescence with an exposure time of 30 seconds.
Anti-Apc6 antibody (ab72136)参考文献
has not yet been referenced specifically in any publications.