The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
Use at 2-5 µg/mg of lysate.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Protein modification; protein ubiquitination.
Belongs to the APC5 family. Contains 4 TPR repeats.
All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg Lane 2 : Whole cell lysate from Hela cells at 15 µg Lane 3 : Whole cell lysate from Hela cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 85 kDa Observed band size: 85 kDa
Exposure time: 3 minutes
Immunoprecipitation - Apc5 antibody (ab72516)
Immunoprecipitation/ Western Blot of Apc5.
Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded).
Lane 2: Control IgG.
ab72516 at 1µg/ml for WB.
Chemiluminescence with an exposure time of 1 minute.