The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
Use at 2-5 µg/mg of lysate.
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
All lanes : Anti-Apc4 antibody (ab72149) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 92 kDa Observed band size: 92 kDa Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Masuda K et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS One10:e0118662 (2015).
Read more (PubMed: 25723520) »
Ma HT & Poon RY Orderly Inactivation of the Key Checkpoint Protein Mitotic Arrest Deficient 2 (MAD2) during Mitotic Progression. J Biol Chem286:13052-9 (2011).
Read more (PubMed: 21335556) »