概述

  • 产品名称
  • 描述
    兔多克隆抗体to Apc4
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 758 and 808 of human Apc4 (NP_037499.2).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the APC4 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANAPC 4 antibody
    • anapc4 antibody
    • Anaphase Promoting Complex Subunit 4 antibody
    • Anaphase-promoting complex subunit 4 antibody
    • APC 4 antibody
    • APC Complex Protein CUT20 antibody
    • APC4 antibody
    • APC4_HUMAN antibody
    • CUT20 antibody
    • Cyclosome subunit 4 antibody
    • LID1 antibody
    see all

Anti-Apc4 antibody 图像

  • All lanes : Anti-Apc4 antibody (ab72149) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-Apc4 antibody (ab72149)参考文献

This product has been referenced in:
  • Masuda K  et al. LATS1 and LATS2 phosphorylate CDC26 to modulate assembly of the tetratricopeptide repeat subcomplex of APC/C. PLoS One 10:e0118662 (2015). Read more (PubMed: 25723520) »
  • Ma HT & Poon RY Orderly Inactivation of the Key Checkpoint Protein Mitotic Arrest Deficient 2 (MAD2) during Mitotic Progression. J Biol Chem 286:13052-9 (2011). WB ; Human . Read more (PubMed: 21335556) »

See all 3 Publications for this product

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