概述

  • 产品名称
  • 描述
    兔多克隆抗体to Apc10
  • 特异性
    This antibody detects a band at about 26kD that corresponds to Apc10.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    GADPKQLERTG

    , corresponding to amino acids 10-20 of Human Apc10.

  • 常规说明
    Expiration date is one (1) year from date of opening.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • 通路
    Protein modification; protein ubiquitination.
  • 序列相似性
    Belongs to the APC10 family.
    Contains 1 DOC domain.
  • Information by UniProt
  • 数据库链接
  • 形式
    It localises to Cytosol and nucleoplasm.
  • 别名
    • ANAPC 10 antibody
    • anapc10 antibody
    • Anaphase promoting complex 10 antibody
    • Anaphase promoting complex subunit 10 antibody
    • Anaphase-promoting complex subunit 10 antibody
    • Apc 10 antibody
    • APC10 antibody
    • APC10_HUMAN antibody
    • Cyclosome subunit 10 antibody
    • DKFZP564L0562 antibody
    • Doc 1 antibody
    • Doc1 antibody
    • Doc1, S. cerevisiae, homolog of antibody
    • OTTHUMP00000220297 antibody
    • OTTHUMP00000220298 antibody
    • OTTHUMP00000220299 antibody
    • OTTHUMP00000220300 antibody
    • OTTHUMP00000220303 antibody
    see all

图片

  • All lanes : Anti-Apc10 antibody (ab3629) at 1/500 dilution

    Lane 1 : Hela nuclear lysates
    Lane 2 : Hela cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate
    Lane 5 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Predicted band size : 21 kDa


    Exposure time : 4 minutes

实验方案

文献

ab3629 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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