Anti-Apc1 (phospho S355)抗体(ab10923)
Key features and details
- Rabbit polyclonal to Apc1 (phospho S355)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Apc1 (phospho S355)抗体
参阅全部 Apc1 一抗 -
描述
兔多克隆抗体to Apc1 (phospho S355) -
宿主
Rabbit -
特异性
Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human Apc1 aa 350-450 (phospho S355) conjugated to keyhole limpet haemocyanin.
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常规说明
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Primary antibody说明
This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10923于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
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说明 |
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WB
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa). |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC1 family.
Contains 4 PC repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 535565 Cow
- Entrez Gene: 475750 Dog
- Entrez Gene: 64682 Human
- Entrez Gene: 17222 Mouse
- Omim: 608473 Human
- SwissProt: E1BAB6 Cow
- SwissProt: E2RLM2 Dog
- SwissProt: Q9H1A4 Human
see all -
别名
- ANAPC 1 antibody
- Anapc1 antibody
- Anaphase promoting complex subunit 1 antibody
see all
图片
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All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 215 kDa
Observed band size: 215 kDa
Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab10923 被引用在 1 文献中.
- Zhang Y et al. miR-29a suppresses IL-13-induced cell invasion by inhibiting YY1 in the AKT pathway in lung adenocarcinoma A549 cells. Oncol Rep 39:2613-2623 (2018). PubMed: 29620222