纯化说明This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
Primary antibody说明This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
1/10000 - 1/40000.
Tested in peptide ELISA. Product has been tested against 0.1 µg of the phosphorylated and non-phosphorylated forms of the immunizing peptide.
功能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the APC1 family. Contains 4 PC repeats.
翻译后修饰Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
Western blot - Apc1 (phospho S355) antibody (ab10923)
All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution
Lane 1 : HeLa S3 whole cell lysate (asynchronous cells) Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine) Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)
Lysates/proteins at 30 µg per lane.
Secondary Goat anti-Rabbit IgG [H&L] at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 215 kDa Observed band size : 215 kDa
Anti-Apc1 (phospho S355) antibody (ab10923)参考文献
has not yet been referenced specifically in any publications.