Anti-Apc1 (phospho S355)抗体(ab10923)

概述

  • 产品名称Anti-Apc1 (phospho S355)抗体
    参阅全部 Apc1 一抗
  • 描述
    兔多克隆抗体to Apc1 (phospho S355)
  • 特异性Less than 1% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is expected to be phospho-specific for S355 of APC1.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide: SRAHSpPALG conjugated to KLH, corresponding to amino acids 351-359 of Human Apc1.

  • 常规说明


    This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This affinity purified antibody is directed against the phosphorylated form of human APC1 at the pS355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Primary antibody说明This is one of a number of novel phosphorylation sites for APC1 identified in Kraft C et al. It may be involved in the regulation of the APC/C.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200 - 1/2000. Detects a band of approximately 215 kDa (predicted molecular weight: 215 kDa).
ELISA 1/10000 - 1/40000.

Tested in peptide ELISA. Product has been tested against 0.1 µg of the phosphorylated and non-phosphorylated forms of the immunizing peptide.

靶标

  • 功能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the APC1 family.
    Contains 4 PC repeats.
  • 翻译后修饰Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANAPC 1 antibody
    • Anapc1 antibody
    • Anaphase promoting complex subunit 1 antibody
    • anaphase-promoting complex 1 (meiotic checkpoint regulator) antibody
    • Anaphase-promoting complex subunit 1 antibody
    • Apc 1 antibody
    • APC1 antibody
    • APC1_HUMAN antibody
    • Cyclosome subunit 1 antibody
    • MCPR antibody
    • Meiotic checkpoint regulator antibody
    • Mitotic checkpoint regulator antibody
    • Protein Tsg 24 antibody
    • Protein Tsg24 antibody
    • Testis-specific gene 24 protein antibody
    • TSG 24 antibody
    • TSG24 antibody
    see all

Anti-Apc1 (phospho S355) antibody 图像

  • All lanes : Anti-Apc1 (phospho S355) antibody (ab10923) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate (asynchronous cells)
    Lane 2 : HeLa S3 whole cell lysate (cells synchronized at G1/S boundary by treatment with thymidine)
    Lane 3 : HeLa S3 whole cell lysate (cells synchronized at M phase by treatment with nocodazole)

    Lysates/proteins at 30 µg per lane.

    Secondary
    Goat anti-Rabbit IgG [H&L] at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 215 kDa
    Observed band size : 215 kDa

Anti-Apc1 (phospho S355) antibody (ab10923)参考文献

ab10923 has not yet been referenced specifically in any publications.

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