应用说明peptide ELISA: 1/80000 (determined against 0.1 µg of the phosphorylated and non-phosphorylated forms of the immunizing peptide). Approximately equivalent staining is seen when both the phosphorylated and non-phosphorylated form of the immunizing peptide are bound to the ELISA plate.
We have tested this antibody on various human tissue lysates and have as yet detected no bands of the appropriate size, however we have seen low background.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
通路Protein modification; protein ubiquitination.
序列相似性Belongs to the APC1 family. Contains 4 PC repeats.
翻译后修饰Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis.