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Synthetic peptide corresponding to APC aa 1-226.
APC is well suited for immunohistochemical and immunofluorescence studies of oligodendrocytes and optic nerves due to the antibody’s staining of the cell body as opposed to the myelinated processes.
Although we list several publications demonstrating mouse reactivity, we have also received conflicting data from researchers demonstrating a lack of mouse reactivity. Therefore, we have removed mouse from our list of reactive species and no longer guarantee the product for this species
Our Abpromise guarantee covers the use of ab16794 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-FrFl||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
ICC/IF image of ab16794 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16794, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC-F formalin-fixed, paraffin embedded human cerebellum with ab16794.
Antibody was used at a concentration of 2.5 mg/ml and sections were pretreated with heat using a pressure cooker prior to staining. Detection with DAB and hematoxylin as a counterstain
Overlay histogram showing HCT116 cells stained with ab16794 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16794, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"