概述

  • 产品名称
  • 描述
    兔多克隆抗体to APBA1
  • 特异性
    Detects APBA1 (Mint 1) from rat brain extract as well as extract from HEK293 cells overexpressing the rat gene.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide corresponding to Rat APBA1 aa 1-17.
    Sequence:

    MNHLEGSAEVEVADEAP


    (Peptide available as ab4980)

  • 阳性对照
    • Rat forebrain postnuclear supernatant.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • Primary antibody说明
    The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 93 kDa).Can be blocked with APBA1 peptide (ab4980).

By Western blot, this antibody detects an ~130 kDa protein representing Mint 1 from rat forebrain postnuclear supernatant.

靶标

  • 功能
    Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
  • 组织特异性
    Brain and spinal cord.
  • 序列相似性
    Contains 2 PDZ (DHR) domains.
    Contains 1 PID domain.
  • 结构域
    Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter protein X11 alpha antibody
    • Adapter protein X11alpha antibody
    • Amyloid beta A4 precursor protein-binding family A member 1 antibody
    • Apba1 antibody
    • APBA1_HUMAN antibody
    • Mint 1 antibody
    • Mint-1 antibody
    • Neuron specific X11 protein antibody
    • Neuron-specific X11 protein antibody
    • Neuronal Munc18 1 interacting protein 1 antibody
    • Neuronal Munc18-1-interacting protein 1 antibody
    • UROP11 antibody
    • x11 antibody
    • X11alpha antibody
    see all

图片



  • Predicted band size : 93 kDa

    Western blot of APBA1 on extract from HEK293 cells overexpressing the rat gene using ab3448.

实验方案

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Thank you for your enquiry. Ab 3448 has been tested for cross-reactivity with rat, and has not yet been tested for cross-reactivity in other species. We do not know if Ab3448 will give a single band on chick brain extract. Should you decide to go a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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