Anti-APAF1抗体(ab53152)

概述

  • 产品名称Anti-APAF1抗体
    参阅全部 APAF1 一抗
  • 描述
    兔多克隆抗体to APAF1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from Human APAF1.

  • 阳性对照
    • Human lung carcinoma tissue; extracts from COLO205.

性能

应用

Our Abpromise guarantee covers the use of ab53152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 141 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • 组织特异性Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • 序列相似性Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • 结构域The CARD domain mediates interaction with APIP.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

Anti-APAF1 antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with ab53152 at a dilution of 1/50-1/100. Right hand image: treated with immunising peptide. Left hand image: untreated.

Anti-APAF1 antibody (ab53152)参考文献

ab53152 has not yet been referenced specifically in any publications.

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I am happy to confirm that this antibody was tested with citrate buffer pH6 as antigen retrieval.



Antigen Retrieval

1. Bring 500 -2000 ml 10 mM citrate buffer (pH6.0) to the boil in a...

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