• 产品名称Anti-APAF1抗体
    参阅全部 APAF1 一抗
  • 描述
    兔多克隆抗体to APAF1
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human APAF1 aa 1158-1177 (C terminal).


    (Peptide available as ab7872)

  • 阳性对照
    • Human heart tissue lysate IF/ICC: HEK293 cell line
  • 常规说明Apoptosis Protease Activating Factor




Our Abpromise guarantee covers the use of ab2000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 20 µg/ml.
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.Can be blocked with APAF1 peptide (ab7872).


  • 功能Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
  • 组织特异性Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
  • 序列相似性Contains 1 CARD domain.
    Contains 1 NB-ARC domain.
    Contains 13 WD repeats.
  • 结构域The CARD domain mediates interaction with APIP.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APAF 1 antibody
    • Apaf-1 antibody
    • APAF_HUMAN antibody
    • Apaf1 antibody
    • Apoptotic peptidase activating factor 1 antibody
    • Apoptotic protease activating factor 1 antibody
    • Apoptotic protease activating factor antibody
    • Apoptotic protease-activating factor 1 antibody
    • CED 4 antibody
    • CED4 antibody
    • KIAA0413 antibody
    see all

Anti-APAF1 antibody 图像

  • ICC/IF image of ab2000 stained HEK293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2000, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-APAF1 antibody (ab2000) at 1 µg/ml

    Lane 1 : Human heart tissue lysate with absence of blocking peptide
    Lane 2 : Human heart tissue lysate with presence of blocking peptide

    Predicted band size : 130 kDa
    Observed band size : 130 kDa
  • Immunohistochemistry of Apaf1 in human skin tissue with Apaf1 antibody at 20 µg/ml.

Anti-APAF1 antibody (ab2000)参考文献

ab2000 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (C57Bl/6 mouse liver)
Loading amount 10 µg
Specification C57Bl/6 mouse liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

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提交于 Oct 19 2011

Anti-Apaf-1 NT (2013) and anti-Apaf-1 CT (2015) were gernerated with peptides selected from the N-terminus and C-terminus of the protein. 888 513 9525