概述

  • 产品名称Anti-AP4抗体
    参阅全部 AP4 一抗
  • 描述
    兔多克隆抗体to AP4
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: GNCGRSFAQW DQLVAHKRVH VAEALEEAAA KALGPRPRGR PAVTAPRPGG , corresponding to a region within internal sequence amino acids 180 - 229 of Human AP4 (NP_037532)

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab83414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 相关性AP4 (Replication initiator 1) is a sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. It binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. AP4 facilitates DNA bending.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • 60 kDa origin specific DNA binding protein antibody
    • 60 kDa replication initiation region protein antibody
    • ATT binding protein antibody
    • DHFR oribeta binding protein RIP60 antibody
    • H_DJ0584D14.12 antibody
    • REPIN1 antibody
    • Replication initiation region protein (60kD) antibody
    • Replication initiator 1 antibody
    • RIP60 antibody
    • Zfp464 antibody
    • Zinc finger protein 464 (RIP60) antibody
    • Zinc finger proten AP4 antibody
    • ZNF464 antibody
    see all

Anti-AP4 antibody 图像

  • Anti-AP4 antibody (ab83414) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

Anti-AP4 antibody (ab83414)参考文献

ab83414 has not yet been referenced specifically in any publications.

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