Anti-AP3B2抗体(ab48269)
Key features and details
- Rabbit polyclonal to AP3B2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-AP3B2抗体
参阅全部 AP3B2 一抗 -
描述
兔多克隆抗体to AP3B2 -
宿主
Rabbit -
特异性
By Western blot this antibody detects a ~119-130 kDa protein representing AP3B2 from brain samples. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human AP3B2 aa 260-274. Mouse sequence is LLEENPEKAFYGSEE
Sequence:LLEENAEKAFYGSEE
(Peptide available asab48404) -
阳性对照
- Human brain samples.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
-
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500. Detects a band of approximately 119-130 kDa (predicted molecular weight: 119 kDa).
|
说明 |
---|
WB
1/500. Detects a band of approximately 119-130 kDa (predicted molecular weight: 119 kDa). |
靶标
-
功能
Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. -
组织特异性
Expression is specific to nervous system. Expressed in nerve terminal and cell body, and is associated with nerve-terminal vesicles. Expression seen in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). -
序列相似性
Belongs to the adaptor complexes large subunit family. -
发展阶段
Expressed from early in development through adulthood. -
细胞定位
Golgi apparatus. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Golgi apparatus. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. - Information by UniProt
-
数据库链接
- Entrez Gene: 8120 Human
- Entrez Gene: 11775 Mouse
- Entrez Gene: 308777 Rat
- Omim: 602166 Human
- SwissProt: Q13367 Human
- SwissProt: Q9JME5 Mouse
- Unigene: 199593 Human
- Unigene: 322894 Mouse
-
别名
- Adapter-related protein complex 3 subunit beta-2 antibody
- Adaptor protein complex AP-3 subunit beta-2 antibody
- Adaptor related protein complex 3 beta 2 subunit antibody
see all
图片
-
Anti-AP3B2 antibody (ab48269) at 1/500 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
A ~50 kDa band is also seen in Rat and Mouse brain tissue, representing the medium chain homolog (p47A) of AP3.
数据表及文件
-
Datasheet download
文献 (1)
ab48269 被引用在 1 文献中.
- Ku YC et al. The transcriptome of utricle hair cell regeneration in the avian inner ear. J Neurosci 34:3523-35 (2014). PubMed: 24599453