Anti-AP3抗体(ab115234)

概述

  • 产品名称Anti-AP3抗体
    参阅全部 AP3 一抗
  • 描述
    山羊多克隆抗体to AP3
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    SPSPQKRYQDTPG

    conjugated to KLH, corresponding to amino acids 686-698 of Human AP3 (NP_003929.4).

  • 阳性对照
    • Human lung (respiratory epithelium) tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab115234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 130 kDa.
ELISA 1/5000 - 1/20000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.
  • 组织特异性Present in all adult tissues examined with the highest levels in skeletal muscle, heart, pancreas and testis.
  • 序列相似性Belongs to the adaptor complexes large subunit family.
    Contains 11 HEAT repeats.
  • 细胞定位Cytoplasm. Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adapter-related protein complex 3 subunit delta-1 antibody
    • adaptor-related protein complex 3, delta 1 subunit antibody
    • ADTD antibody
    • AP 3 complex delta subunit antibody
    • AP-3 complex subunit delta antibody
    • AP-3 complex subunit delta-1 antibody
    • ap3d1 antibody
    • AP3D1_HUMAN antibody
    • Delta 1 subunit of adaptor-related protein complex 3 antibody
    • Delta Adaptin antibody
    • Delta-adaptin antibody
    • hBLVR antibody
    • Subunit of putative vesicle coat adaptor complex AP 3 antibody
    see all

Anti-AP3 antibody 图像

  • Human lung, respiratory epithelium tissue (formalin-fixed, paraffin-embedded) stained with ab115234 at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-AP3 antibody (ab115234)参考文献

ab115234 has not yet been referenced specifically in any publications.

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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ab85075 has not been tested in IP either, nor in mouse. Thus, we do not know if and how well the antibody might work in that species and application. In either case, you would be eligible for the testing discount pr...

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I am very sorry that the antibody is not working well in human samples either.

I'd be happy to replace, credit or refund the antibody.

We carry 4 AP3 antibodies, but none of them has been so far ...

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I have received a WB image with human samples. It is attached to the email.

Approximately 20 ug of HeLa cell extracts were loaded,the membrane was incubated with a 1:500 dilution of the antibody. The lo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"