Anti-AP2S1抗体[EPR2696] (ab92380)


  • 产品名称Anti-AP2S1抗体[EPR2696]
    参阅全部 AP2S1 一抗
  • 描述
    兔单克隆抗体[EPR2696] to AP2S1
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human AP2S1.

  • 阳性对照
    • Fetal brain lysate, HeLa cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR2696
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab92380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP 1/10.
Flow Cyt 1/200. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.
    • 序列相似性Belongs to the adaptor complexes small subunit family.
    • 细胞定位Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Adapter-related protein complex 2 sigma subunit antibody
      • Adaptor protein complex AP 2 subunit sigma antibody
      • Adaptor protein complex AP-2 subunit sigma antibody
      • Adaptor protein complex AP2 subunit sigma antibody
      • Adaptor related protein complex 2 sigma 1 subunit antibody
      • AI043088 antibody
      • AP 17 antibody
      • AP 2 complex subunit sigma 1 antibody
      • AP-2 complex subunit sigma antibody
      • AP17 antibody
      • AP17 delta antibody
      • AP2 complex subunit sigma 1 antibody
      • Ap2s1 antibody
      • AP2S1_HUMAN antibody
      • CLAPS 2 antibody
      • CLAPS2 antibody
      • Clathrin adaptor complex AP2, sigma subunit antibody
      • Clathrin adaptor protein AP17 antibody
      • Clathrin assembly protein 2 small chain antibody
      • Clathrin associated/assembly/adaptor protein small 2 antibody
      • Clathrin associated/assembly/adaptor protein small 2, 17-KD antibody
      • Clathrin associated/assembly/adaptor protein, small 2 (17kD) antibody
      • Clathrin coat assembly protein AP17 antibody
      • Clathrin coat associated protein AP17 antibody
      • Clathrin coat-associated protein AP17 antibody
      • HA2 17 kDa subunit antibody
      • MGC62945 antibody
      • Plasma membrane adaptor AP 2 17 kDa protein antibody
      • Plasma membrane adaptor AP-2 17 kDa protein antibody
      • Plasma membrane adaptor AP2 17 kDa protein antibody
      • Sigma adaptin 3b antibody
      • Sigma2 adaptin antibody
      • Sigma2-adaptin antibody
      see all

    Anti-AP2S1 antibody [EPR2696] 图像

    • All lanes : Anti-AP2S1 antibody [EPR2696] (ab92380) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 17 kDa

    Anti-AP2S1 antibody [EPR2696] (ab92380)参考文献

    ab92380 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (endothelial)
    Specification endothelial
    Fixative Paraformaldehyde
    Permeabilization Yes - triton 0.1%
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

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    提交于 Jun 28 2011