重组Anti-AP2M1 (phospho T156)抗体[EPR4700] (ab109397)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4700] to AP2M1 (phospho T156)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-AP2M1 (phospho T156)抗体[EPR4700]
参阅全部 AP2M1 一抗 -
描述
兔单克隆抗体[EPR4700] to AP2M1 (phospho T156) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Rat C6, Human HeLa, and Mouse NIH/3T3 whole cell lysates treated with calyculin A.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4700 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109397于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 50 kDa. |
靶标
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功能
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling. -
序列相似性
Belongs to the adaptor complexes medium subunit family.
Contains 1 MHD (mu homology) domain. -
细胞定位
Cell membrane. Membrane > coated pit. AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV. - Information by UniProt
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数据库链接
- Entrez Gene: 1173 Human
- Entrez Gene: 11773 Mouse
- Entrez Gene: 116563 Rat
- Omim: 601024 Human
- SwissProt: Q96CW1 Human
- SwissProt: P84091 Mouse
- SwissProt: P84092 Rat
- Unigene: 518460 Human
see all -
别名
- Adapter-related protein complex 2 mu subunit antibody
- Adaptin mu 1 antibody
- Adaptin-mu2 antibody
see all
图片
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All lanes : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution (Purified)
Lane 1 : C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by RIPA lysis method
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by RIPA lysis method
Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa1%SDS Hot lysis method is preferred to enhance the signal.
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Lanes 1 & 3 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution (Purified)
Lane 2 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate prepared by 1%SDS Hot lysis method
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method
Lane 3 : C6 (Rat glial tumor glial cell) treated with 100ng/ml calyculin A for 1 hour whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with alkaline phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa -
Lanes 1 & 3 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution (Purified)
Lane 2 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM calyculin A for 30 minutes whole cell lysate. Membrane then treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa -
Lane 1 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution (Purified)
Lanes 2-3 : Anti-AP2M1 (phospho T156) antibody [EPR4700] (ab109397) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared by 1%SDS Hot lysis method
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 50nM calyculin A for 2 hours whole cell lysate prepared by 1%SDS Hot lysis method. Membrane then treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab109397 被引用在 8 文献中.
- Liu Q et al. Dysregulation of the AP2M1 phosphorylation cycle by LRRK2 impairs endocytosis and leads to dopaminergic neurodegeneration. Sci Signal 14:N/A (2021). PubMed: 34315807
- Heaton GR et al. Sequential screening nominates the Parkinson's disease associated kinase LRRK2 as a regulator of Clathrin-mediated endocytosis. Neurobiol Dis 141:104948 (2020). PubMed: 32434048
- Agajanian MJ et al. WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop. Cell Rep 26:79-93.e8 (2019). PubMed: 30605688
- Wrobel AG et al. Temporal Ordering in Endocytic Clathrin-Coated Vesicle Formation via AP2 Phosphorylation. Dev Cell 50:494-508.e11 (2019). PubMed: 31430451
- Wang C et al. The Serine/Threonine Kinase AP2-Associated Kinase 1 Plays an Important Role in Rabies Virus Entry. Viruses 12:N/A (2019). PubMed: 31905947
- Partlow EA et al. A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex. Elife 8:N/A (2019). PubMed: 31464684
- Beacham GM et al. NECAPs are negative regulators of the AP2 clathrin adaptor complex. Elife 7:N/A (2018). PubMed: 29345618
- Hollopeter G et al. The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex. Elife 3:N/A (2014). PubMed: 25303366