RabMAb

Anti-AP2 gamma抗体[EP2693Y] (ab75992)

概述

  • 产品名称
    Anti-AP2 gamma抗体[EP2693Y]
    参阅全部 AP2 gamma 一抗
  • 描述
    兔单克隆抗体[EP2693Y] to AP2 gamma
  • 特异性
    The immunogen used for this product shares 88% homology with cadherin EGF LAG seven-pass G-type receptor 1. Cross-reactivity with this protein has not been confirmed experimentally
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminus of human AP2 gamma.

  • 阳性对照
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100000 - 1/200000. Detects a band of approximately 49 kDa.
IP 1/60.
Flow Cyt 1/200. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能
      Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC.
    • 序列相似性
      Belongs to the AP-2 family.
    • 结构域
      The WW-binding motif mediates interaction with WWOX.
    • 翻译后修饰
      Sumoylated on Lys-10; which inhibits transcriptional activity.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activating enhancer binding protein 2 gamma antibody
      • Activating enhancer-binding protein 2 gamma antibody
      • AP2-gamma antibody
      • AP2C_HUMAN antibody
      • AP2g antibody
      • AP2gamma antibody
      • ERF 1 antibody
      • ERF1 antibody
      • Estrogen receptor factor 1 antibody
      • hAP 2g antibody
      • hAP2g antibody
      • TFAP 2C antibody
      • TFAP 2G antibody
      • Tfap2c antibody
      • TFAP2G antibody
      • Transcription factor AP 2 gamma (activating enhancer binding protein 2 gamma) antibody
      • Transcription factor AP 2 gamma antibody
      • Transcription factor AP-2 gamma antibody
      • Transcription factor ERF 1 antibody
      • Transcription factor ERF-1 antibody
      • Transcription factor ERF1 antibody
      see all

    图片

    • Anti-AP2 gamma antibody [EP2693Y] (ab75992) at 1/200000 dilution + MCF-7 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Observed band size : 49 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized MCF-7cells using ab75992 (red) or a rabbit IgG (negative) (green).

    文献

    This product has been referenced in:
    • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24469049) »

    See 1 Publication for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human ESC)
    Gel Running Conditions
    Reduced Denaturing (4-12% Tris Bis)
    Loading amount
    500000 cells
    Specification
    Human ESC
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Mr. Timothy Hunt

    Verified customer

    提交于 Aug 23 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton-X100 in PBS
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Sep 29 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册