Anti-AP2 gamma/TFAP2C抗体[3B5] (ab110635)
Key features and details
- Mouse monoclonal [3B5] to AP2 gamma/TFAP2C
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
概述
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产品名称
Anti-AP2 gamma/TFAP2C抗体[3B5]
参阅全部 AP2 gamma/TFAP2C 一抗 -
描述
小鼠单克隆抗体[3B5] to AP2 gamma/TFAP2C -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: PC-7, A431, mouse placenta, and RAW 264.7 cell lysates; ICC/IF: HeLa, MCF-7, and U251 cells; IHC-P: Human breast cancer;
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99.85% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3B5 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50.
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WB |
1/100 - 1/1000. Predicted molecular weight: 49 kDa.
Block with BSA only (not milk). |
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ICC/IF |
1/50 - 1/500.
|
说明 |
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IHC-P
1/50. |
WB
1/100 - 1/1000. Predicted molecular weight: 49 kDa. Block with BSA only (not milk). |
ICC/IF
1/50 - 1/500. |
靶标
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功能
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. -
序列相似性
Belongs to the AP-2 family. -
结构域
The WW-binding motif mediates interaction with WWOX. -
翻译后修饰
Sumoylated on Lys-10; which inhibits transcriptional activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7022 Human
- Entrez Gene: 21420 Mouse
- Omim: 601602 Human
- SwissProt: Q92754 Human
- SwissProt: Q61312 Mouse
- Unigene: 473152 Human
- Unigene: 3629 Mouse
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别名
- Activating enhancer binding protein 2 gamma antibody
- Activating enhancer-binding protein 2 gamma antibody
- AP2-gamma antibody
see all
图片
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in Hela Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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All lanes : Anti-AP2 gamma/TFAP2C antibody [3B5] (ab110635) at 1/50 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Mouse placenta cell lysate
Lane 4 : RAW 264.7 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 49 kDa -
Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with ab110635 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in U251 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
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Immunofluorescent analysis of AP2 gamma/TFAP2C using ab110635 shows staining in MCF-7 Cells. AP2 gamma/TFAP2C staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab110635 at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a Goat Anti-Mouse DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab110635 被引用在 1 文献中.
- Benchetrit H et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell 24:983-994.e7 (2019). PubMed: 31031139