Anti-AP2 beta抗体[AP2b 1G6/2] (ab18114)

概述

  • 产品名称Anti-AP2 beta抗体[AP2b 1G6/2]
    参阅全部 AP2 beta 一抗
  • 描述
    小鼠单克隆抗体[AP2b 1G6/2] to AP2 beta
  • 经测试应用适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Truncated AP-2 beta protein prepared from bacteria.

性能

应用

Our Abpromise guarantee covers the use of ab18114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
IHC-Fr
  • 应用说明ELISA: Use at an assay dependent dilution.
    IHC-Fr: 1/1000. Use perfusion fixed tissue (see Abreview).
    WB: Use at an assay dependent dilution. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.
    • 疾病相关Defects in TFAP2B are the cause of Char syndrome (CHAR) [MIM:169100]. CHAR is an autosomal dominant disorder characterized by patent ductus arteriosus (PDA), facial dysmorphism and hand anomalies.
    • 序列相似性Belongs to the AP-2 family.
    • 翻译后修饰Sumoylated on Lys-21; which inhibits transcriptional activity.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Activating enhancer binding protein 2 beta antibody
      • Activating enhancer-binding protein 2-beta antibody
      • AP 2B antibody
      • AP2 B antibody
      • AP2-beta antibody
      • AP2B antibody
      • AP2B_HUMAN antibody
      • AP2beta antibody
      • MGC21381 antibody
      • OTTHUMP00000039925 antibody
      • TFAP 2B antibody
      • Tfap2b antibody
      • Transcription factor AP 2 beta antibody
      • Transcription factor AP-2-beta antibody
      • Transcription factor AP2 beta antibody
      see all

    Anti-AP2 beta antibody [AP2b 1G6/2] 图像

    • ab18114 at 1/1000 staining perfusion fixed adult rat brain tissue sections by IHC-Fr. Adult rat was perfused intracardially with paraformaldehyde 4% in PB 0.2M. The brain was post-fixed in the same fixative for 24 hours. Sections were cryoprotected with sucrose 20% and later frozen in OCT. Sections were incubated in free floating 12h with the primary antibody (1/1000) and revealed with secondary antibody conjugated with Alexa Fluor ® 488 (1/2000).

      The staining obtained is restricted to the cytoplasm and consists of a small and thin punctate staining (white arrows). The picture shows the staining obtained at the level of the spinal cord using the X40 objective.

      See Abreview

    Anti-AP2 beta antibody [AP2b 1G6/2] (ab18114)参考文献

    ab18114 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry.

    I am sorry to confirm that ab18114AP2 beta antibody [AP2b 1G6/2] has notbeen tested in IHC-P .

    It is likely that the antibody will still work in IHC-P, although we cannot provide our guarantee for this ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Adult rat brain)
    Specification Adult rat brain
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Dr. Sophie Pezet

    Verified customer

    提交于 Jun 30 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"