概述

  • 产品名称Anti-AP2 alpha抗体[EPR2688(2)]
    参阅全部 AP2 alpha 一抗
  • 描述
    兔单克隆抗体[EPR2688(2)] to AP2 alpha
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human AP2 alpha.

  • 阳性对照
    • JAR and RAW264.7 cell lysates; Human breast carcinoma tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EPR2688(2)
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle.
  • 疾病相关Defects in TFAP2A are the cause of branchiooculofacial syndrome (BOFS) [MIM:113620]; also known as branchial clefts with characteristic facies, growth retardation, imperforate nasolacrimal duct, and premature aging or lip pseudocleft-hemangiomatous branchial cyst syndrome. BOFS is a rare autosomal dominant cleft palate craniofacial disorder with variable expressivity. The major features include cutaneous anomalies, ocular anomalies, characteristic facial appearance (malformed pinnae, oral clefts), and, less commonly, renal and ectodermal (dental and hair) anomalies.
  • 序列相似性Belongs to the AP-2 family.
  • 结构域The WW-binding motif mediates interaction with WWOX.
  • 翻译后修饰Sumoylated on Lys-10; which inhibits transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activating enhancer binding protein 2 alpha antibody
    • Activating enhancer-binding protein 2-alpha antibody
    • Activator protein 2 antibody
    • AP 2 transcription factor antibody
    • AP 2alpha antibody
    • AP-2 antibody
    • AP-2 transcription factor antibody
    • AP2 antibody
    • AP2 Transcription Factor antibody
    • AP2-alpha antibody
    • AP2A_HUMAN antibody
    • AP2TF antibody
    • BOFS antibody
    • FLJ51761 antibody
    • TFAP 2 antibody
    • TFAP 2A antibody
    • TFAP2 antibody
    • TFAP2A antibody
    • Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha) antibody
    • Transcription factor AP-2-alpha antibody
    • Transcription factor AP2 alpha antibody
    see all

Anti-AP2 alpha antibody [EPR2688(2)] 图像

  • ab108311 staining AP2 alpha in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde, permiabilised with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/140. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • All lanes : Anti-AP2 alpha antibody [EPR2688(2)] (ab108311) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : JAR cell lysate
    Lane 3 : RAW264.7 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 48 kDa
  • ab108311 at 1/100 dilution staining AP2 alpha in paraffin embedded Human breast carcinoma tissue.
  • ab108311 at 1/250 dilution staining AP2 alpha in HeLa cells.

Anti-AP2 alpha antibody [EPR2688(2)] (ab108311)参考文献

This product has been referenced in:
  • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24469049) »

See 1 Publication for this product

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